Cat no.: LN001
Production Name: NICK Label DNA Probe Kit
Kit component :
Ø Nick Translation Mix for .5x conc.(50% glycerol (v/v) and DNA Polymerase I) DNase I)
Size: 25Test
Note:
(1)Nick Translation kit can be used in probe of FISH, Northern probe, Sourthern probe ).
(2)Denaturation of the template is not required before nick translation
(3)The probe labeled in the range of 200 to 500 nucleotides.
(4)Need to analyze the fragment length distribution of labeled probe before stopping the reaction with 0.5M EDTA and heat 65℃ 10.
(5) For research only. Not for use in diagnosis.
(6) PCR products should be purified
STORAGE:
• at -20 °C, Do not freeze. Not more than 12 months.
• Avoid storing reagents or working solution in strong direct light.
Intended Use and Application:
(1)Sample DNA material: Supercoiled and linearized plasmid DNA, supercoiled and linearized cosmid DNA, purified PCR products.
(2)The Nick Translation Mix for in situ probes is designed for direct fluorophore-labeling of in situ probes. Fluorescein-12-dUTP*, Dig-dUTP, Bio-dUTP and tetramethyl-rhodamine-6-dUTP*
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